The Three Lions travel to Chisinau from Lithuania on Monday afternoon, after basing themselves in Kaunas since Friday’s 1-0 victory against the Baltic state.
And Southgate knows that he’ll be without the midfield pair of James Ward-Prowse and Nat Chalobah for the game in Tiraspol, after they both picked up their second cautions of the campaign in that game to serve one-match bans.
Moldova v England
UEFA European U21 Championship
Group One Qualifier
5pm (BST), Tuesday 9 September 2014
Sheriff Sports Complex, Tiraspol
"They’ll miss the next game, but we’ve got a squad with us so it’s fine," said Southgate, whose side are already confirmed as Group One winners ahead of the match with second-placed Moldova.
"I’m pleased with what they’ve both done and I want to look at different players against Moldova anyway."
With the Euro play-off draw next Friday consisting of the ten group winners and the four best second-placed teams, Moldova will be chasing victory to give themselves a chance of progressing.
But the Three Lions Head Coach is hoping to take a look at his own options in the match too, with a 21-man group having been together since last Sunday preparing for the double-header.
Southgate admitted: "We’ll try one or two different things, but it will be a different test because they’ll play with three at the back and try to come at us a bit more.
"That will be a good tactical test for us and they’re the types of challenge that we might face moving forward in the play-offs.
"It will be a different sort of game."
The Three Lions squad arrive in the Moldovan capital Chisinau on Monday evening, where they will stay ahead of the game.
They will then travel on Tuesday to Tiraspol, which is the second-biggest city in Moldova but also the ‘capital’ of what is the internationally unrecognised state of Transnistria.
