The FA Women’s Premier League Cup Final will be staged at the Lamex Stadium, home of League Two Stevenage FC, on Sunday 7 May.
The showpiece will be an all-London affair between holders Tottenham Hotspur and 2015 winners Charlton Athletic.
FA Women’s Premier League chairman Carol West said: “The FA Women's Premier League Cup is one of the most illustrious women's competitions with a long and distinguished history.
“Since the restructure of The FA WPL in 2014 we have been keen to uphold the tradition of the Cup and are thrilled that Stevenage will host this year's Final.
“The Lamex Stadium will provide a fantastic platform for what will be a real cracker of a game.
“Charlton Athletic and Tottenham Hotspur are real heavyweights in the women's game with a rivalry that will definitely entertain.”
Tickets are priced £5 for adults, including a matchday programme, £1 for under-10s and £2.50 for under-16s and senior citizens.
Tickets can be bought directly from the Stevenage website or from the turnstiles on the day of the game.