England Women will travel to Portugal on Tuesday 8 October as part of an international double-header that will begin with Brazil three days before in Middlesbrough.
The fixture will be played at the Bonfim Stadium, home of Primeira Liga club Vitória FC in Setúbal, approximately 40km south of Lisbon.
It will be the first meeting between the two nations since their group stage match at the 2017 UEFA EURO in the Netherlands, when goals from Toni Duggan and Nikita Parris helped England to a 2-1 victory.
“This will be another important match for us as we work to be ready for EURO 2021," said Lionesses head coach Phil Neville.
"Like our recent fixtures in Belgium and Norway we are looking for competitive games that will challenge us and the work we're doing on the training pitch. Portugal will be a different kind of test and one we will relish.”
After the October matches, England will take on Germany at Wembley Stadium on Sunday 9 November.