The FA Women's Cup reaches its Third Round stage next month as the ten FA WSL 2 sides join the action.
Following a busy weekend of action, the draw was made at Wembley Stadium on Monday.
And there were two ties out of the hat that feature rivals from the second tier going head-to-head.
The FA Women's Cup
Third Round Proper draw
Ties will take place on Sunday 1 February
Winning clubs will receive £400
Six-time Cup winners Doncaster Rovers Belles, who finished runners up in the inaugural FA WSL 2 season, have been drawn at home against London Bees.
The Bees ended their campaign at the bottom of the division, and suffered two heavy defeats to the Belles.
Meanwhile, FA WSL 2 champions Sunderland travel to Aston Villa in their Third Round tie on 1 February and will be looking to add to their two victories during the league season.
After a 3-2 extra-time win over Cardiff City, Charlton Athletic, winners of The Cup in 2005, have been paired with Wolverhampton Wanderers of the Premier League Northern Division.
Brighton & Hove Albion currently top the Southern Division table and they beat league rivals West Ham United 6-2 to line up an away trip to FA WSL 2 side Watford.
But the weekend's biggest winners - Sheffield, who won 8-0 at Leicester City - will travel down to Essex to take on FA Premier League South East Division One side C&K Basildon.
The 16 Third Round ties are scheduled for Sunday 1 February.
1 Oxford United v Coventry City
2 Blackburn Rovers or Liverpool Marshall Feds v Portsmouth
3 Doncaster Rovers Belles v London Bees
4 Yeovil Town v Lewes
5 Radcliffe Olympic v Durham Women’s
6 Bradford City v FC Reedswood
7 Aston Villa v Sunderland
8 Morecambe v Stoke City
9 Carshalton Athletic v Keynsham Town
10 Millwall Lionesses v Lowestoft Town
11 Crystal Palace v Reading Women
12 Charlton Athletic v Wolverhampton Wanderers
13 C&K Basildon v Sheffield
14 Eastbourne Town v Derby County
15 Tottenham Hotspur v Preston North End
16 Watford v Brighton & Hove Albion